Regulatory

Part:BBa_K2610005

Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans   Group: iGEM18_Leiden   (2018-10-09)


pLamB

LamB is involved in the transport of maltose and maltodextrins by encoding the channel maltoporin in E.coli. LamB gene expression is repressed by a low pH. (Maurer et al, 2004)

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the lamB promoter is moderately activated by apramycin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None